· Research Interests ·  

• Analytical methods for extremely complex samples and their applications based on chromatography-mass spectrometry.

• Metabolomics analytical platform and its application in disease biomarker discovery, synthetic biology, plant functional genome, metabolic networks analysis and reconstruction.

• Development of new exposomics methods and their application in environmental and adverse health effects.

• Single cell and spatial metabolomics in precision medicine: focused on major diseases such as cancer, diabetes, liver disease etc.

  · 代 表 性 工 作 ·  

 1、代谢组学平台技术及其应用 

 Method Development and Applications of Metabolomics 

1.1 高覆盖、高灵敏、高通量新方法

High coverage, high sensitivity and high throughput method

通过发展新型多维液相色谱-质谱联用系统以及拟靶向代谢组学技术，构建了一整套代谢组学精准测量技术体系，实现了代谢组高效分离、规模化定性和精准定量。

By developing novel multi-dimensional liquid chromatography-mass spectrometry system and pseudotargeted metabolomics technology, a set of precise metabolomics measurement technologies has been constructed, achieving efficient metabolomics separation, large-scale qualitative analysis and precise quantification.

1)High-coverage metabolomics analysis based on multi-dimensional chromatography-mass spectrometry technology

Anal Chim Acta, 2017, 966, 34; Anal Chem 2017, 89, 12902; Anal Chem, 2017, 89, 1433

2) High-precise metabolomics analysis based on pseudotargeted technology

Anal Chem, 2013, 85, 8326; Anal Chem, 2015, 87, 5050; Anal Chem, 2018, 90, 7608; 

Anal Chem, 2018, 90, 11401; Anal Chem, 2020, 92, 6043; Nature Protocols, 2020, 15, 2519

1.2 基于人工智能和大数据的代谢组规模化鉴定方法

Scalable Metabolite Annotation Based on AI and Big Data

LC–HRMS单次分析可采集数千至上万个代谢物特征。然而，受限于参考谱图库的规模，现有搜库方法仅能识别约1.8%~18%的代谢特征，严重制约了代谢组数据的深度解析。为此，系统研发了涵盖算法创新、数据库构建及大数据挖掘的多层次代谢组注释体系，显著提升了代谢组规模化注释能力。

LC-HRMS enables determination of thousands of metabolite features per run, yet traditional methods identify only 1.8%~18% due to spectral library limitations, hindering deep metabolomics insights. We established multi-level annotation strategies that significantly enhance large-scale metabolome coverage.

1)Heuristic-Based Metabolomics Annotation

                                                          Anal Chem, 2018, 90, 9, 5712                                                Anal Chem, 2017, 89, 23, 12902

2)LC-MS/MS Database-Based Annotation

                                     J Food Compos Anal, 2023,123, 105523                         Anal Chem, 2022, 94, 24, 8561；2024, 96, 19190

3) Molecular Networking-Based Annotation

                               Anal Chem, 2023, 95, 11603；2024, 96, 1444；2025        中国发明专利：ZL202011407735.8, ZL202011407735.8

                                                   doi.org/10.1021/acs.analchem.5c00314                                   软件著作权证书登记：2021SR0667243                     

1.3 单细胞代谢组学 / 亚细胞代谢组学

Single-cell metabolomics / subcellular metabolomics

通过研制新型复合电离源及微流控进样系统，结合高分辨质谱，发展了高覆盖、高通量单细胞代谢组学技术。通过研制高性能磁性亲和材料，高灵敏亚细胞代谢组学分析成为可能。

By developing composite ionization sources and microfluidic injection system, combined with high-resolution mass spectrometry, high-coverage and high-throughput single-cell metabolomics technologies have been developed; By developing high-performance magnetic affinity materials, highly sensitive subcellular metabolomics techniques have been developed.

1) High-coverage and high-throughput single-cell metabolomics

                                                 Advanced Science, 2024, e2306659                                         Anal Chem, 2024 96, 14935

2) High-coverage and highly sensitive metabolomics methods for mitochondria and exosomes

                                     Anal Chem, 2021, 93, 11099                                                Anal Chem, 2020, 92, 15497

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 2、食品安全与暴露组学 

 Food safety and exposomics 

2.1 食品化学风险筛查新技术研究

New screening methods for chemical hazards in foods

研发食品化学风险筛查新技术，实现复杂食品基质中已知及未知风险的高效精准检测。

Develop new screening methods for chemical hazards in foods to enable efficient and accurate detection of both targeted and untargeted hazards within complex food matrices.

1）Novel omics-based screening approaches were established for comprehensive detection of unidentified chemical hazards in complex food matrices.

                                               Anal Chem, 2016, 88, 8870                                              Anal Chem, 2018, 90, 8454

2）Non-targeted screening methods based on fragmentation characteristics enable the precise identification of unknown hazards and their transformation products.

                                                               Food Chem, 2022, 369, 130928                                                 Talanta, 2024, 275, 126116

3）Non-targeted screening methods based on intelligent algorithms have been established to enable high-throughput detection and identification of unknown chemical hazards.

                                     Anal Chim Acta, 2024, 1287, 342116                               Environ Pollut, 2025, 365 ,125338

2.2 暴露组学新方法与慢病关联研究

New exposome methods and chronic disease associations

研发生物样本中暴露组筛查新技术，开展大规模人群暴露特征及其慢病风险研究。

Develop new screening methods for the exposome in biological samples and investigate the associations between exposome profiles and chronic disease risks in large-scale population studies.

1）This study developed a 2D-LC-HRMS-based method for screening 1210 exogenous chemicals in human serum, providing a powerful tool to investigate the impact of environmental exposures on human health.

Environ Pollut, 2023, 331, 121914

2）Through serum biomonitoring covering 267 chemicals of 5,696 healthy individuals and chronic disease patients in China, we found that region is the key factor influencing exposure levels, while organochlorine pesticides and perfluoroalkyl substances are linked to multiple chronic diseases.

Nat Commun, 2024, 15, 2268

2.3 全暴露组学与慢病分子机制研究

Exposome-wide molecular mechanisms of chronic diseases

使用全暴露组学分析方法，系统解析大规模人群暴露特征与慢性疾病发生、发展的内在分子机制。

Use new exposome-wide methods and systematically elucidate the intrinsic molecular mechanisms linking population-wide exposure characteristics and progression of chronic diseases.

1）A unified method enabling single-injection dual-mode detection was developed for untargeted metabolomics and targeted exposomics analysis.

Anal Chem, 2025, 97, 3996

2）Through the study of exogenous compounds and chronic diseases, we found significantly positive association between PFASs exposure and hyperuricemia.

Environ Int, 2022, 158, 106919

3）We found, through studying the toxicity effects and mechanisms of PFECA, that PFO5DoDA can cause liver homeostasis disruption through a glucocorticoid suppressive effect.

J Hazard Mater, 2023, 447, 130831

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 3、疾病与功能代谢组学 

 Disease and functional metabolomics 

3.1代谢组学技术平台

Platform of metabolomics

1）Standard operation procedure of metabolomics

Clin Chem, 2013, 59, 833; Clin Chem, 2018, 64, 810; Anal Chim Acta, 2018,1037, 293

2）Integrated analytical platforms with high coverage of the metabolome

3）High-quality data acquisition and large-scale metabolic profiling methods for metabolomics

Anal Chem, 2013, 85, 8326; J Proteome Res, 2015, 14, 906; Anal Chem, 2015, 87, 5050;

J Chromatogr A, 2016, 1437, 127; Anal Chem, 2016, 88, 2234; Nat Protoc, 2020, 15, 2519;

Anal Chem, 2022, 94(24), 8561

3.2功能代谢组学平台及典型应用

Functional Metabolomics Platform and Classic Applications

HCC metabolic reprogramming and biomarkers	Key molecular mechanisms of dysregulated lipid metabolism in HCC
Cancer Res, 2013, 73, 4992        Hepatology, 2018, 67, 662	Nat Commun, 2022, 13, 2187
Novel metabolic intervention strategies for liver failure treatment	Selective therapeutics for IDH1-mutated glioma by targeting glutathione synthesis pathway
Adv Sci, 2020, 7, 1902996	Proc Natl Acad Sci U S A, 2020, 117(18), 9964

3.3菌群及其相关代谢物的研究

Research on Gut Microbiota and Associated Metabolites

1)Novel High-Coverage LC-HRMS Method for Detecting 1,250 Gut Microbiota-Related Metabolites

Anal Chem, 2024, 96, 2206

2)Resistant starch targets gut microbiota to treat nonalcoholic fatty liver disease and obesity

Cell Metab, 2023, 35, 1530                                                       Nat Metab, 2024, 6,578

3)Maturation of the gut metabolome during the first year of life in humans

Gut Microbes, 2023, 15, 2231596                                             Homepage: www.402.dicp.ac.cn